Abstract:To study the sequence and expression characteristics of Lipin1 (lpin1) gene of Megalobrama amblycephala and the effect of muscle injury on its expression, in this study, the temporal and spatial expression of lpin1 gene and its expression change after muscle injury were detected by fluorescent quantitative PCR and whole embryo in situ hybridization. The results showed that the ORF sequence of lpin1 gene was 2694 bp, encoding 897 amino acids. Functional domain analysis showed that this protein had highly conserved Lipin-N, Lipin-mid and LNS2 (Lipin/Ned1/Smp2) domains. Phylogenetic tree analysis showed that Lpin1 was closely related to Cyprinus carpio and Danio rerio. The results of quantitative PCR showed that lpin1 was expressed at all stages during the embryonic development, and the expression level was the highest in the muscle of adult M. amblycephala. Whole embryo in situ hybridization further revealed that lpin1 was specifically expressed in the head and body segments during muscle functioning stage and heartbeating stage. Compared with the uninjured group, the lpin1 expression was significantly increased at 48 h after mild injury and 216 h after severe injury, the pax7 expression was significantly increased at 144 h after mild injury and 96 h after severe injury. We conclude that lpin1 gene may play an important role in muscle injury and repair of M.amblycephala.