Based on the data of the labor force of relocation households in J County,Hubei Province,binary Logit regression model is used to explore the impact of different types of family burdens on poor labor force and the gender differences of this impact. The results show that overall,different types of family burdens have different impacts on the employment of the poor labor force,among which,the burden of support for the elderly promotes the employment of the poor labor force,and the burden of disease hinders the employment of the poor labor force. In terms of gender,there are gender differences in the impact of family burdens on the employment of the poor labor force. Women are more likely to be affected by family burdens than men. Therefore,the research points out that the rural public service construction should be improved to reduce the impact of family burden on employment. And the opportunities of home employment should be increased to reduce the conflict between employment and family and promote the full employment of the poor labor force.