In order to solve the problem of unbalanced regional development and regional poverty，my country has implemented the East-West Poverty Alleviation Cooperation Strategy which reflects China’s institutional advantages.Since poverty alleviation，the two parties have mainly carried out assistance work in several aspects，such as industrial cooperation，labor cooperation，talent support，financial support，and working together to achieve a well-off society.The poverty alleviation cooperation center was moved down，and the assistance relationships such as county-county pairing，town-township pairing，village-village pairing，and village-enterprise pairing were explored.The East-West Poverty Alleviation Cooperation Policy has been further developed and deepened in many aspects such as poverty alleviation subjects，poverty alleviation resources，and poverty alleviation fields.It has surpassed the meaning of poverty alleviation and development wor'k itself，and has produced positive spillover effects on my country’s regional development.This not only It is embodied as a kind of social responsibility，but also as a strategy to achieve a win-win situation for the whole society.East-West cooperation will still play an important role in solving the problem of unbalanced and insufficient development between regions，improving the quality and efficiency of regional economic development，and steadily advancing the rural vitalization strategy.Based on the target positioning of the rural vitalization strategy，this paper explains and explores the connotation，positioning and innovation of deepening East-West cooperation in the new development stage，which is not only the realistic need to consolidate and expand poverty alleviation，but also provides a reference for the policy direction and implementation path of East-West cooperation under the rural vitalization strategy.