To break the innovation bottleneck of comprehensively promoting rural revitalization， the key is to overcoming low overall efficiency of innovation system caused by fragmentation， low-level duplication and low conversion rate. The particularity of agricultural and rural development in China has resulted in the division of the innovation system into three relatively independent phases: production， transformation and extension. The reason for this problem is the self-circulation and shortening of innovation chain caused by poor connection of three stages and logical differentiation of different subjects as well as the inability of small farmers to express their innovation needs and the high cost of innovation service supply, leading to an imbalance in innovation supply and demand. Without establishing an intrinsic connection between innovation supply and demand and emphasizing the input of innovation supply, more resources may be wasted. The rapid development of entrepreneurship has formed a large number of subjects with endogenous demand for innovation and rural comprehensive revitalization of talents， which helps to break the self-cycle， smooth the circulation channel of innovation elements， and promote the balanced development of agricultural and rural innovation. It is an effective mechanism to strengthen driving force of innovation by linking entrepreneurship and innovation and making up for the weaknesses of the innovation system with high-quality entrepreneurship. It is suggested to integrate innovation-oriented and innovative entrepreneurship into the agricultural and rural innovation system， coordinate livelihood and innovative entrepreneurship， establish incubation and service system for innovative entrepreneurship， optimize rural business and development environment， and promote integrated development of entrepreneurship and innovation.