We use general self-efficacy questionnaire (GSES) to analyze gender differences of agricultural university students’ career self-efficacy.The results show that there is gender difference in agricultural university students’career self-efficacy and boys’career self-efficacy is higher than girls’.Gender differences of agricultural university students' career self-efficacy have social cause and students’ cause.We should take effective measures to improve female students’ career self-efficacy in agricultural university.We should strengthen women’s confidence,perfect statutes to opposite employment discrimination,and pertinently reinforce employment training and consulting.Students should seek reasons correctly,and strive to expand professional fields.