The regulatory effects of intracerebroventricular （ICV） injection and deprived feed of histidine or valine on the feeding of Chinese perch （Siniperca chuatsi） were studied to explore the direct signal perception of the central nervous system and the indirect signal perception of the peripheral tissues. In the ICV injection test，the control group was injected with phosphate buffered saline （PBS），and the experimental group was injected with histidine or valine or simultaneously with an inhibitor of general control non-derepressible-2 （GCN2） GCN2iB. The changes in food intake of Chinese perch at 1 h and 4 h after injection were detected. In the feed deprivation test，the control group was fed with amino acid complete feed and the experimental group was fed with deprived feed of histidine or valine. The changes in food intake，GCN2 signaling pathway-related factors and appetite genes were detected. The results showed that the injection of either histidine or valine significantly reduced the food intake of Chinese perch compared with the control group （P<0.05）. Simultaneous injection of GCN2iB and histidine restored the food intake of Chinese perch to normal levels. Compared with the control group，the intake of deprived feed of histidine or valine was significantly decreased （P<0.05）. Moreover，the mRNA expression of activating transcriptional factor 4 （atf4），a key activating transcription factor downstream of GCN2 signaling pathway，was significantly down-regulated （P<0.05） in deprived feed of histidine or valine. The mRNA expression level of the appetite factor neuropeptide Y （npy） in the hypothalamus was significantly decreased （P<0.05）. However，there were no significant changes in the mRNA expression levels of agouti-related protein （agrp），pro-opiomelanocortin （pomc），and amphetamine-regulated transcript （cart） genes. In conclusion，both histidine and valine can inhibit the feeding behavior of Chinese perch via ICV administration and feed deprivation. The changes of feeding behavior of Chinese perch are related to the GCN2 signaling pathway. The deprived feed of histidine or valine might result in the anorexia by down-regulating the expression of appetite-stimulating factor npy gene.