The XAG P20-2019 quadcopter UVA was used to spray foliar fertilizer on Acanthopanax trifoliatus to study the feasibility and optimal combination of working parameters for spraying foliar fertilizer on Acanthopanax trifoliatus with unmanned aerial vehicle （UAV）. The orthogonal experiment with three factors and three levels was designed to investigate the effects of the concentration of spraying， the speed of flight and the height of working on the quality of spraying， the yield of Acanthopanax trifoliatus and the quality of leaf. The results showed that the speed of flight and the height of working had significant effects on the quality of spraying， and the density of droplet deposition had a strong positive correlation with the amount of droplet deposition. The effects of the concentration of spraying， the speed of flight and the height of working on the yield and quality of leaf in Acanthopanax trifoliatus were significant. The density and the amount of droplet deposition was 188.212/cm2 and 0.269 μg/cm2 when the concentration of spraying， the speed of flight and the height of working was 5 times the concentration recommended，1.0 m/s and 2.0 m. At this time， the effect of spraying foliar fertilizer on Acanthopanax trifoliatus with UAV was the best， with a 27.46% increase in the yield of Acanthopanax trifoliatus and a 33.23% increase in the content of chlorophyll in Acanthopanax trifoliatus compared with that of the control. It is indicated that it is feasible to use UAV to spray foliar fertilizer with high concentration on Acanthopanax trifoliatus. It will provide references for using the same type of UVA to spray foliar fertilizer.