The characteristics of carbon emission and carbon sequestration under different farming models of rice-duck-crayfish were compared with those under the traditional farming model of single rice cropping and rice-crayfish farming.The rice variety Huamoxiang 5，the crayfish（Procambarus clarkii），and duck variety Wuqin No.10 were used to conduct a field experiment at the Shuangshui Shuanglü Institute Base of Huazhong Agricultural University in Jingzhou，Hubei Province.The effects of different farming models on the methane （CH?） emission，nitrous oxide （N?O） emission，global warming potential （GWP），greenhouse gas emission intensity （GHGI），and carbon sequestration potential in paddy fields were studied.The results showed that the CH? emissions，GWP，and GHGI under the farming model of rice-duck-crayfish reduced by 28.1% to 32.1%，27.0% to 30.7%，23.0% to 26.7%，while the carbon sequestration increased by 2 089 to 2 569 kg/hm2 compared to that under the traditional model of single rice cropping.The CH? emissions，GWP，and GHGI under the farming model of rice-duck-crayfish reduced by 18.6% to 23.1%，17.9% to 22.2%，and 18.7% to 22.0%，while the carbon sequestration increased by 616 to 1 096 kg/hm2 compared to that under the rice-crayfish farming model.It is indicated that the farming model of rice-duck-crayfish can reduce carbon emissions and increase the level of carbon neutrality in rice paddy fields，with the farming model of rice-duck-crayfish in large field blocks having the best effects on the reduction of emissions and the sequestration of carbon.