To enhance the stress resistance of Procambarus clarkii and improve the economic and ecological benefits of its aquaculture by identifying suitable feed additives，this study selected 360 P.clarkii［（13.59±0.25） g］ and randomly divided them into four treatment groups.The subjects were fed a basal diet （control，CN），a basal diet supplemented with 0.5% Bacillus subtilis （BS），0.5% sodium humate （HNa），or a combination of 0.5% sodium humate +0.5% B. subtilis （BS+HNa），respectively，to investigate the effects of sodium humate and B. subtilis supplementation on the growth performance，antioxidant capacity，and immunoenzyme activity of P. clarkii.The results showed that，regarding growth performance，the feed conversion ratio in the BS，HNa and BS+HNa groups was significantly lower than that in the CN group.Conversely，the protein efficiency ratio in the BS，HNa，and BS+HNa groups was significantly higher than that in the CN group.Additionally，the feed conversion ratio in the BS+HNa group was significantly lower than that in the BS and HNa groups.The weight gain rate，specific growth rate，and protein efficiency ratio in the experimental groups were significantly higher than those in the CN group.Regarding antioxidant property，the activities of catalase （CAT） and total antioxidant capacity （T-AOC） in the hemolymphatic and hepatopancreas tissues of the BS+HNa group were significantly higher than those in the other groups.Additionally，the malondialdehyde （MDA） content was significantly lower than that in the other groups，and the superoxide dismutase （SOD） level was significantly higher than that in the CN group.Moreover，sodium humate and B. subtilis exhibited significant interaction in reducing MDA levels in shrimp.Regarding immunoenzyme activity，the alkaline phosphatase （AKP） and acid phosphatase （ACP） activities activity levels in the hemolymph and hepatopancreas of the BS，HNa and BS+HNa groups were significantly higher than those in the CN group.Furthermore，the activity levels in the BS+HNa group were significantly greater than those in the BS and HNa groups.These results indicate that both individual and combined supplementation with 0.5% sodium humate and 0.5% B. subtilis both improved the growth performance，antioxidant capacity，and immune performance of P. clarkii，with the combined supplementation showing superior effects compared to individual theatment.Therefore，the combined use of sodium humate and B. subtilis is recommended.