To comprehensively evaluate the applicability of black rice and black rice anthocyanin extract in the feed of Procambarus clarkii，450 juvenile crayfish were randomly allocated into five groups.These groups were fed diets supplemented with 0 （control group，CON），200 g/kg black rice （BR200），400 g/kg black rice （BR400），200 mg/kg anthocyanin extract （AC200），or 400 mg/kg anthocyanin extract （AC400） for 8 weeks.The effects of different forms and supplementation levels of black rice anthocyanins in feed on growth performance，antioxidant capacity，immune response，and intestinal health were investigated.The results revealed that： （1） The BR400 group achieved the highest weight gain rate （WGR） and specific growth rate （SGR），showing no significant difference from AC400，and both were significantly higher than those of the control group.（2） Antioxidant enzyme activities in the hemolymph，hepatopancreas，and intestine were elevated across all treatment groups.AC400 exhibited the highest total antioxidant capacity （T-AOC） and superoxide dismutase （SOD） activity in both the hemolymph and hepatopancreas，with no significant difference observed compared to BR400.Notably，BR200 displayed the highest intestinal T-AOC and SOD activity，significantly surpassing both AC400 and CON groups.（3） Acid phosphatase （ACP） and alkaline phosphatase （AKP） activities in hepatopancreas and intestine were significantly elevated across all treatment groups.BR400 exhibited the highest levels of polyphenol oxidase （PPO），ACP，and AKP activities in the hemolymph.（4） AC400 demonstrated the highest intestinal amylase activity，while AC200 showed the highest trypsin activity.Additionally，crypt depth in all treatment groups was significantly greater than in CON.These findings indicate that dietary supplementation with either 400 g/kg of black rice or 400 mg/kg of black rice anthocyanin extract effectively enhances growth performance，antioxidant capacity，immune function，and digestive efficiency in P. clarkii.Optimal supplementation levels are recommended at 400 g/kg of black rice or 400 mg/kg of black rice anthocyanin extract.