Humic substances， as a natural organic matter commonly present in soil and sediment， is crucial in the processes of biogeochemical cycling and energy flux within ecosystems.The active functional groups including quinones contained in the molecular structure of humic acid endow humic acid with redox properties， making it play a key role in the transformation of environmental pollutants and the improvement of soil fertility.Accurately evaluating the redox capacity of humic substances is the foundation for the in-depth elucidation of its environmental behavior and functional mechanisms.At present， the main methods for evaluating the redox capacity of humic substances include redox titration， electrochemical techniques， and microbial assays.This article reviewed the principles， experimental protocols， and application scope of oxidation-reduction titration， electrochemical techniques， and microbial assays， and deeply analyzed the advantages and limitations of each method in evaluating the redox capacity of humic substances.The results showed that the redox titration is simple to operate， but its quantitative accuracy is limited by the way of determining endpoint.Electrochemical techniques have high sensitivity but are susceptible to external interference from environments.Microbial assays can simulate natural conditions well but have a longer cycle of analyses.The emerging trends for evaluating the redox capacity of humic substances are discussed in terms of developing new electrochemical sensors， optimizing in-situ measurement techniques， and implementing multi-modal analytical strategies.It will offer a theoretical framework for selecting appropriate methods for evaluating the redox capacity of humic substances from diverse sources and establishing a robust scientific foundation for promoting the application of humic substances in remediating environment and boosting agricultural productivity.