Chili pepper was used to conduct a pot experiment to study the effects of Trichoderma harzianum and dolomite，and the combined application of both on the improvement of acidic soils. A two-factor and three-level orthogonal experiment （Trichoderma harzianum applied at 0 g/pot，3 g/pot and 6 g/pot，and dolomite applied at 0 g/kg，3 g/kg，and 6 g/kg） was designed to result in 9 treatment groups including H₀S₀，H₃S₀，H₆S₀，H₀S₃，H₃S₃，H₆S₃，H₀S₆，H₃S₆ and H₆S₆. The results showed that the combined application of Trichoderma harzianum and dolomite significantly increased soil pH. The H₆S₆ had the highest content of organic matter in soil. The combined application of Trichoderma harzianum and dolomite increased the content of alkaline-hydrolyzable nitrogen in soil，and the high-dose combination of Trichoderma harzianum with dolomite （H₆S₃，H₆S₆） significantly increased the content of available phosphorus. The combined application of high-dose dolomite and high-dose Trichoderma harzianum significantly increased the content of total nitrogen and urease activity in soil. Compared with the control group （H₀S₀），the content of total nitrogen in the H₆S₆ treatment increased by 213.50% and the activity of urease in the H₆S₆ treatment enhanced by 61.97%. In terms of the absorption of nutrient by chili peppers，the content of total nitrogen and total phosphorus in the fruits in H₃S₃ treatment was the highest，significantly increased by 32.32% and 292.37% compared with that in H₀S₀ （CK）. It is indicated that the combined application of Trichoderma harzianum and dolomite can effectively improve the nutrient status in acidic soils and the activity of enzyme in acidic soils，and promote the absorption of nutrient by chili peppers，with the H₆S₆ treatment having the best performance in terms of comprehensive benefits. It will provide a scientific basis for the improvement of acidified soil.