57 main rice varieties planted in the middle and lower reaches of the Yangtze River were used to conduct hydroponic culture experiment under the low temperature and the toxicity of iron（Fe²⁺） and manganese（Mn²⁺） to screen the adaptive rice varieties in cold-waterlogged paddy fields in this area.The selected tolerant and sensitive varieties were used to analyze the differences in the growth phenotype，root architecture，content of iron and manganese，and the activity of antioxidant enzyme.The results showed that there were no significant differences in most growth indexes and physiological and biochemical indexes in cold tolerant or toxicity of iron and manganese tolerant varieties compared to that in sensitive varieties under control conditions.The growth indexes and physiological and biochemical indexes in cold tolerant or toxicity of iron and manganese tolerant varieties were better than those in sensitive varieties under treatment.The survival rate，aboveground biomass，content of chlorophyll b，and activity of POD in cold tolerant varieties was significantly higher than that in cold sensitive varieties under low temperature treatment，while the content of MDA aboveground in cold tolerant varieties was significantly lower than that in cold sensitive varieties.The plant height，total root length，root volume and surface area，root tip number，and aboveground dry weight of Fe²⁺ tolerant varieties was significantly higher than that of ++Fe²⁺ sensitive varieties under ++Fe²⁺ treatment.The content of iron in the root of ++Fe²⁺ tolerant varieties was significantly lower than that of ++Fe²⁺ sensitive varieties，and the activity of SOD and POD in the root and aboveground parts of ++Fe²⁺ tolerant varieties was significantly higher than that of ++Fe²⁺ sensitive varieties.The plant height，primary root length，root surface area，root and shoot dry weight in Mn²⁺ tolerant varieties were higher than that in ++Mn²⁺ sensitive varieties under ++Mn²⁺ treatment.The content of Mn²⁺ in root and shoot of ++Mn²⁺ tolerant varieties was significantly lower than that of ++Mn²⁺ sensitive varieties，while the activity of SOD and POD in the shoot of ++Mn²⁺ tolerant varieties was higher than that of ++Mn²⁺ sensitive varieties.Several varieties such as Quanyousimiao（5#），Hongxiangyousimiao（9#），Jiafengyou No.2（19#），Quanliangyou No. 534（33#），Weiliangyou No. 534（34#）and Hualiangyou No. 2817（53#）with high-yield and multi-resistance were obtained through Venn diagram analysis and composite stress screening，which can be used as candidate varieties for popularizing and planting in cold-waterlogged paddy fields.