To systematically evaluate the Al-toxicity of maize varieties and to establish an efficient screening system，45 hybrid maize varieties were selected to conduct Al-toxicity stress trials in this study.The experiment was designed in a dual-treatment mode：basic acidic soil （pH 5.15） and high-aluminum-toxic acidic soil to reveal the resistance mechanism of the varieties by comparing the differences in stress.During the critical growth stage of maize at the 3-leaf stage，the research team systematically measured 12 morpho-physiological indexes，including stem diameter，plant height，and SPAD value，and quantified the level of resistance by introducing the aluminum toxicity tolerance coefficient （C_Alt）.In this study，we used a multidimensional analysis method to construct an evaluation system，firstly，we extracted the characteristic indexes by dimensional reduction of principal component analysis，combined with the affiliation function method to calculate the comprehensive resistance evaluation value （D value），and then we used the systematic clustering to classify the varieties into five resistance levels （among which extremely resistant D≥0.75，extremely sensitive D≤0.25），and then we screened out the extremely resistant varieties of aluminum toxicity，such as GY14022，EHD1，QKY900，and HY1702，and the aluminum toxicity resistant varieties of GY14022，EHD1，QKY900 and HY1702，and the aluminum toxicity resistant varieties of GY14022，EHD1，QKY900 and HY1702.HY1702，and extremely sensitive varieties TR1818，JF86 and ZY303.It was found that aluminum stress significantly inhibited the development of maize seedlings （P<0.001），the plant height and biomass decreased by 5.37% and 17.65%，respectively，and there was a significant correlation between the resistance indexes （P<0.05），which verified the reliability of the evaluation system.Finally，through the double validation of gray correlation and stepwise regression，the five core screening indexes of root fresh weight，total dry weight，SPAD value，total fresh weight and root length were confirmed.In conclusion，aluminum toxicity stress severely inhibited the growth of maize at seedling stage，and there were significant differences in acid tolerance among different maize varieties.The comprehensive analysis screened root fresh weight，total dry weight，SPAD，total fresh weight and root length as the key indexes for screening of aluminum toxicity resistance of maize at seedling stage.