The interleukin-17 receptor （IL-17R） family in mammals regulates inflammatory responses by mediating the signaling of the IL-17 cytokine. Partial cDNA sequences of five Pf_IL-17R genes （Pf_IL-17RA to Pf_IL-17RE） were cloned from yellow catfish （Pelteobagrus fulvidraco，Pf） to study the potential role of IL-17R gene in the immune response to pathogenic infection in fish. The molecular characteristics of the sequence，expression profiles of tissue，and changes in expression of five genes in yellow catfish infected with Edwardsiella ictaluri and stimulated with immunostimulants were analyzed. The results showed that the open reading frame （ORF） of the five Pf_IL-17R genes including Pf_IL-17RA，Pf_IL-17RB，Pf_IL-17RC，Pf_IL-17RD，Pf_IL-17RE was 2 430 bp，1 590 bp，2 106 bp，2 235 bp，and 2 307 bp，encoding 809 amino acids （aa），529 aa，701 aa，744 aa，and 768 aa，respectively. The results of analyzing the sequence of aa showed that the five Pf_IL-17R genes in yellow catfish had high sequence similarity with homologous genes of other teleosts，among which the sequence similarity with homologous genes of channel catfish was the highest. The results of analyses with Real time fluorescence quantitative PCR showed that the five Pf_IL-17R genes exhibited different profiles of mRNA in healthy yellow catfish，but the levels of their expression were higher in the blood，gonads，and gills. The levels of mRNA expression of five Pf-IL-17R genes were significantly upregulated in the gills，skin，and head kidney of yellow catfish infected with Edwardsiella ictaluri，while the expression levels of Pf_IL-17RB，Pf_IL-17RC，Pf_IL-17RD and Pf_IL-17RE mRNAs were significantly down-regulated in the spleen （P<0.05 or P<0.01）. Moreover，the levels of mRNA expression of four Pf_IL-17R genes （with the exception of Pf_IL-17RC） were induced in the isolated peripheral blood leukocytes （PBLs） of yellow catfish stimulated with lipopolysaccharide （LPS），phytohemagglutinin （PHA） and polyinosinic-polycytidylic acid （Poly I：C）. It is indicated that the five Pf_IL-17R genes may play important roles in the immune response of yellow catfish to pathogen infection.