To investigate the carbon emission and carbon sequestration characteristics of different rice-duck-shrimp farming models compared to single rice cropping and traditional rice-crayfish farming models, a field experiment was conducted at the Double Water and Double Green Research Base of Huazhong Agricultural University in Jingzhou, Hubei Province. The experiment used rice variety Huamo Xiang No. 5, the crayfish Procambarus clarkii, and duck variety Wuqin No. 10 as experimental materials to study the effects of different farming models on methane (CH?), nitrous oxide (N?O) emissions, Global warming potential (GWP), greenhouse gas emission intensity (GHGI), and carbon sequestration potential in paddy fields. The results showed that compared to the traditional single rice cropping model, the rice-duck-crayfish model reduced CH? emissions, GWP, and GHGI in paddy fields, significantly increasing the carbon neutrality effect. Compared to single rice cropping, the rice-duck-crayfish model reduced CH? emissions by 28.1% to 32.1%, GWP by 27.0% to 30.7%, and GHGI by 23.0% to 26.7%, while increasing carbon sequestration by 2089 to 2569 kg/hm2. Compared to the rice-crayfish farming model, the rice-duck-crayfish model reduced CH? emissions by 18.6% to 23.1%, GWP by 17.9% to 22.2%, and GHGI by 18.7% to 22.0%, while increasing carbon sequestration by 616 to 1096 kg/hm2. This study indicates that the rice-duck-crayfish model can reduce carbon emissions in paddy fields and enhance the carbon neutrality level of paddy fields, with the Rice-duck-crayfish large field block model showing the best carbon reduction and sequestration effects.