饲料中添加黑米花青素对克氏原螯虾生长、抗氧化能力、免疫机能和肠道健康的影响
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1.华中农业大学;2.华中农业大学水产学院

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中央高校自主创新基金项目(2662024SCPY008; 2662022SCYJ003); 湖北省洪山实验室重大研发项目(2021hszd002);2023-2024省级小龙虾产业链奖补资金项目


Effects of black rice anthocyanins on growth performance, antioxidant capacity, immune function and intestinal health of Procambarus clarkii
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    摘要:

    为探究饲料中添加黑米花青素对克氏原螯虾生长性能、抗氧化能力、免疫机能和肠道健康的影响,试验选取克氏原螯虾450只,随机分为5组,每组3个重复,每个重复30只虾。饲粮中添加两种来源花青素:黑米(BR组)和黑米提取物(AC组),分别添加200 mg/kg和400 mg/kg,记为CON、BR200、BR400、AC200和AC400,试验期8周。结果表明:饲料中添加400 mg/kg黑米花青素显著提升了试验虾的增重率、特定生长率和成活率(P<0.05),同时显著提高了试验虾肝胰腺免疫相关酶PPO、AKP和ACP的活性水平(P<0.05);在消化能力方面,饲料中添加400 mg/kg黑米花青素显著提高了肠道淀粉酶活性(P<0.05),其绒毛高度与隐窝深度的比值显著高于对照组(P<0.05);并且花青素处理组其肠道的胰蛋白酶活性均显著高于对照组(P<0.05)。然而,BR400和AC400试验组之间差异并不显著(P>0.05);与对照组相比,花青素处理组的试验虾血淋巴的T-AOC活性水平均显著高于对照组(P<0.05);并且血淋巴和肠道的AKP、ACP等免疫相关酶活性水平均显著提高(P<0.05)。综上所述,饲料中添加黑米或添加黑米花青素提取物均能够有效地提高克氏原螯虾生长、抗氧化、免疫和消化性能,建议添加量为400mg/kg。

    Abstract:

    In order to explore the effects of adding black rice anthocyanins in the diet on growth performance, antioxidant capacity, immune function and intestinal health of Procambarus clarkii, 450 Procambarus clarkii were selected and randomly divided into 5 groups with 3 replicates per group and 30 shrimps per replicate. Two anthocyanin sources, black rice (BR group) and black rice extract (AC group), were supplemented with 200 mg/kg and 400 mg/kg respectively, and they were recorded as CON, BR200, BR400, AC200 and AC400. The experiment lasted for 8 weeks. The results show that: The results showed as follows: adding 400 mg/kg black rice anthocyanin significantly increased the weight gain rate, specific growth rate and survival rate of shrimp (P<0.05), and significantly increased the activity levels of hepatopancreas immune-related enzymes PPO, AKP and ACP of shrimp (P<0.05). In terms of digestibility, adding 400 mg/kg black rice anthocyanin significantly increased intestinal amylase activity (P<0.05), and the ratio of villus height to crypt depth was significantly higher than that of control group (P<0.05). The intestinal trypsin activity of anthocyanin treatment group was significantly higher than that of control group (P<0.05). However, there was no significant difference between BR400 and AC400 groups (P>0.05); Compared with control group, T-AOC activity level of hemolymph in anthocyanin treated group was significantly higher than that in control group (P<0.05). The activity levels of immune-related enzymes such as AKP and ACP in hemolymph and intestinal tract were significantly increased (P<0.05). In conclusion, adding black rice or black rice anthocyanin extract in the diet can effectively improve the growth, antioxidant, immune and digestive properties of Procambarus clarkii. The recommended supplemental level is 400 mg/kg.

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  • 收稿日期:2024-09-30
  • 最后修改日期:2025-03-04
  • 录用日期:2025-04-08
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