In April, 2002, an outbreak of imported koi occurred in a farm in Guangdong Province with the mortality of 80% upward. In order to investigate the aetiology, the suspension of brain, kidney, spleen and liver were inoculated into fish cell lines of CO,
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刘荭,史秀杰,高隆英,江育林. Study on the Aetiology of Koi Epizootic Disease Using the Method of nested-Polymerase Chain Reaction Assay (nested-PCR)[J]. Jorunal of Huazhong Agricultural University,2002,21(5).