Spleenil cells generated from anti-idiotype antibody were obtained from SPF chicken immunized with McAb against bursin. Total RNA was isolated from these cells and cDNA was synthesized by reverse transcription. Two sets of oligonucleotide primer were desi
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邬向东,曲悦,袁汉英,谌南辉. Construction of Gene Library of the Bursin Anti-idiotype Antibody Variable Region[J]. Jorunal of Huazhong Agricultural University,2003,22(4).