Methylation variations of artificial F4 and F10 generations of Raphanus sativus×Brassica alboglabra allopolyploid were examined with MSAP.The results showed that the methylation levels of F4,F10 were 30.61% and 33.10%,respectively.Twelve different models of methylation variations were detected,and these models could be divided into 5 types,with 41.71% variable methylation sites.The methylation levels of F4 and F10 after 5-azacytidine treatment were 20.67% and 25.75%,respectively.The results showed that after 5-azacytidine treatment,methylation variations of F10 were stronger than those of F4.