The recombinant prokaryotic expression vector pTYB1orf216 with the intein tag was constructed and transformed into E.coli ER2566.The optimized protein expression induction condition in E.coli was 0.8 mmol/L IPTG (isopropy-β-D-thiogalacto side) at 15 ℃ for 15 hours.A band with 85 ku of molecular weight was observed on SDSPAGE,and the protein was mainly existing in the soluble composition.This protein was isolated and purified through affinity chromatography,and then polyclonal antibody was prepared.The results of Western blot indicated that the prepared polyclonal antibody was with high specificity.