The additional components of NS and NSP strains of Guangdong isolate of banana bunchy top virus(BBTV) were cloned by polymerase chain reaction(PCR) and sequenced. They were denoted BT-NS and BT-NSP respectively. The length of both BT-NS and BT-NSP was 1 094 nucleotides. DNA and amino acids sequences deduced from the DNA fragments were analyzed by basic local alignment search tool(BLAST), the homologies shared 97.3% and 98.4% respectively.However,genome organization of the two additional components genome organization had familiar with regions,putative encoding replication initiation proteins motif. The replicase amino acid sequences associated with BBTV were analyzed by phylogenetic tree. The additional component BBTV NS/NSP isolate and BBTV-S1 were grouped together, while BBTV DNA in Guangdong isolate was outgrouped to BBTV NS/NSP strains additional component.