The inhibition activities of tea saponin against Pyricularia grisea,Rhizoctonia solani,Sclerotinia sclerotiorum,Penicillium italicum,Glomerella lagenaria,Fusarium oxysporumr,Glomerella ciugulata,Colletotrichum musae,Phytophthora parasitica,Fusarium oxysporum,Bipolaris maydis and Peronophythora litchi were studied by mycelium growth rate inhibition assay. The results showed that tea saponin had significant antifungal activity. After treatment with 1 000 μg/mL, the inhibition rates of tea saponin against Pyricularia grisea,Penicillium italicum,Glomerella lagenaria,Sclerotinia sclerotiorum and Bipolaris maydis were 100.00%, 100.00%, 89.12%, 87.27% and 83.13% respectively. The EC50 values against Pyricularia grisea and Penicillium italicum were 20.15 μg/mL and 11.39 μg/mL respectively. Tea saponin had no activity against Fusarium oxysporum with the EC50 value exceeding 2 000 μg/mL. On the other hand,tea saponin had higher antifungal activities against Pyricularia grisea than sulfur tricyclazole,and the inhibition rate against Sclerotinia sclerotiorum was not significantly different betweent tea saponin and procymidone by toxicity comparison in laboratory.