A new PCB-degrading rhizobacterium strain named A1 was isolated from the long-term PCBs-contaminated alfalfa rhizospheric soil by enrichment culture. Through morphological observation and sequence analysis of 16S rDNA, the strain was identified as Pandoraea norimbergensis. GC-MS was used to determine the ability of resting cells degradation of PCBs, and it was found that the strain could totally remove 56.7% of the Aroclor1242 in 72 hours. The bphA1 gene core，encoding the catalytic site of the large subunit of biphenyl dioxygenase，was sequenced and its third structure was also predicted. It showed that the nucleic acid sequence of bphA1 gene had 100% homology compared with Pandoraea sp.JB1,and the third structure of protein had 99.37% identity compared with Comamonas testosteroni B356.