Maize seeds imported from Thailand were tested for Maize chlorotic mottle virus (MCMV) with DAS-ELISA. Among 6 varieties, Sample 5 was positive and others were negative. The result of RT-PCR also showed that Sample 5 was positive. The sequence of the RT-PCR product was determined. This sequence was aligned with 23 sequences of other MCMV isolates and a phylogenetic analysis was completed. The result illustrated that this isolate had short genetic distance to Chinese or Thai isolates and clustered in the same group. 100 seeds of sample 5 were detected for MCMV with DAS-ELISA method and 2 seeds were positive,so the detection rate of MCMV was 2%.