A pot experiment was conducted to determine the effects of application of molybdenum (Mo) and selenium (Se) on the yield,contents of Mo and Se in Chinese cabbage,and contents of soil-available Mo and Se during the two successive planting periods.The results showed that the yield was significantly increased by application of Mo and Se in the first harvest,while yield was not significantly increased by application of Mo and Se in the second harvest.Both application of Mo and Se could prompt the Mo uptaking of Chinese cabbage during the two successive planting periods.Soil-available Mo maintained high level during the two successive planting periods,and application of Mo could meet the needs of producing Mo-rich cabbage during the two successive planting periods.Though both application of Mo and Se could prompt Se uptaking of Chinese cabbage during the two successive planting periods,water-soluble Se and exchangeable Se were decreased after the first harvest and application of Se only could meet the needs of producing Se-rich cabbage in the first harvest.