According to the RNASeq mycelium growth and sclerotia formation stage,was selected to study in B. cinerea.The transcript level of BC1G_03293 during sclerotial formation stage was higher than that during mycelium growth stage.The BC1G_03293 of 229 aa signal peptide on the N end and no known conserved domain.The knockout mutants ΔBC1G_032932 and ΔBC1G_032934 were obtained using the homologous recombination method.The complemented transformants ΔBC1G_032932C2 and ΔBC1G_032932C3 were also by Agrobacterium tumefaciensmediated transformation technique.The deletion of BC1G_03293 did not affect the growth,pathogenicity and formation of sclerotia.The conidia production of the knockout mutants obviously decreased and was approximately 45% of the wild type strain.And the conidia production of the complemented transformants recovered obviously.Our research results suggested that BC1G_03293 is involved the regulation of conidiation of B. cinerea.