Cloning and expression profile analysis of odorant receptor gene in Sitotroga cerealella
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    Abstract:

    In the present study,the fulllength cDNA of the odorant receptor coreceptor gene was cloned from the antennae of Sitotroga cerealella (named ScerOrCo) by using reverse transcription polymerase chain reaction (RTPCR) and rapid amplification of cDNA ends (RACE).Sequence analysis revealed that the transcript consisted of 1 876 nucleotides and the open reading frame was 1 422 bp,encoding a peptide of 437 amino acids with 7 putative transmembrane domains.The deduced amino acid sequence of ScerOrCoshared the highest similarity with the olfactory receptor of Mythimna separate (87%).The results of quantitative real time RTPCR showed that the ScerOrCo mRNA was expressed specifically in adult antennae and had no difference between female and male (P>0.05).

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应益益,马敏,陆艳,刘灿,李雪刚,杨凤连. Cloning and expression profile analysis of odorant receptor gene in Sitotroga cerealella[J]. Jorunal of Huazhong Agricultural University,2018,37(1):46-51.

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History
  • Received:July 31,2017
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  • Online: January 02,2018
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