In this study,effect of γ-aminobutyric acid (GABA) and GABA-A receptor antagonist on food intake and glucose metabolism in Chinese perch (Siniperca chuatsi) was investigated.Three groups including intracerebroventricular (ICV) injection of saline and DMSO (control,D),DMSO and 125 μg GABA (DG),and DMSO,125 μg GABA and 20 μg antagonist bicuculline (DGB) were conducted.Food intake was significantly decreased in the DGB group at 0.5 h and 2 h postinjection compared with the D (control) group.Furthermore,the mRNA levels of npy and agrp were decreased significantly,which was coincident with lower food intake in Chinese perch.Blood glucose content was significantly decreased at 0.5 h post injection,but the mRNA levels of cs,pc and pfk1 were not significant changed.The result of co-injection of GABA with bicuculline indicated that GABA acts as an orexigenic factor and further research about GABA on glucose metabolism is needed in Chinese perch.