Degenerated primers were designed according to the conserved regions of NBSLRR domain from known plant resistance genes. Then these primers were used for amplification of the NBSLRR analogs from Lycopersicon esculentum. Sequence analysis showed that 5 fragments contained the resistance gene analogs,two of which have the ORF region and conserved NBSLRR domain. F58 (MG860907) shared 94% identity with the resistance genes of Mi1, and F520 (MG860908) shared 96% identity with the resistance genes of Hero. The quantitative realtime PCR results showed that expression of F58 and F520 was changed after Meloidogyne incognita infection,suggesting that F58 and F520 might be related with nematode infection.