Molecular cloning and analysis of bmp2a and bmp4 promoters in yellow catfish Pelteobagrus fulvidraco
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    Abstract:

    To explore the function of the bmp2a and bmp4 genes and regulatory networks of the related transcription factors in yellow catfish Pelteobagrus fulvidraco, RLM5′RACE method was used to identify the transcription start site (TSS) of bmp2a and bmp4. Then, the promoters of bmp2a and bmp4 were cloned by hiTAILPCR method and a cluster of putative binding sites of several transcription factors were identified by bioinformatics analysis. The results showed that the TSS of bmp2a and bmp4 were located at 391 bp and 351 bp upstream of the coding sequence, respectively. Then 1 830 bp and 1 962 bp upstream of the TSS of bmp2a and bmp4 were cloned and key binding sites of several transcription factors, such as AP1, SP1, GATA1, CREB, PPARγ, SOX5, SOX6 and SOX9, were predicted, suggesting that these transcription factors may play crucial roles in the transcriptional regulation of the bmp2a and bmp4 genes.

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邰志鹏,徐异桓,张电光,谭肖英. Molecular cloning and analysis of bmp2a and bmp4 promoters in yellow catfish Pelteobagrus fulvidraco[J]. Jorunal of Huazhong Agricultural University,2019,38(1):91-96.

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History
  • Received:June 30,2018
  • Revised:
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  • Online: January 03,2019
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