This study was conducted to investigate the function of the SREBP1 gene inMegalobrama amblycephala. The SREBP1 gene was cloned by RTPCR,and its expression were analyzed using qRTPCR. Bioinformatics analysis showed that the cDNA coding sequence (CDS) of SREBP1 was 3 426 bp (GenBank Accession number:MH633449),with an open reading frame (ORF) encoding 1 141 amino acids. The M. amblycephala SREBP1 protein shared high similarity (74%~99%) with Salmo salar,Danio rerio,Ctenopharyngodon idella,Cyprinus carpio and Sinocyclocheilus grahami. Conserved domain analysis showed that the SREBP1 protein has the bHLHZip domains,which belong to the SREBPs family. Expression analysis showed that the SREBP1 expression leveled was the lowest at the optic vesicle stage and highest at the hatching stage during embryonic development of M. amblycephala. For juvenileM. amblycephala,the SREBP1 gene expressed highest in the brain,followed by the eye,liver,heart,fat tissue,kidney and gill. For adultM. amblycephala,theSREBP1 gene expressed highest in the brain,followed by the gonad,muscle,liver,eye,while expressed relatively low in the kidney,spleen and heart. The tissue expression pattern of the SREBP1 gene was different between the juvenile and adult M. amblycephala.