In order to confirm that CRISPR/Cas9 gene editing technology based on lentivirus could be applied to the editing of chicken genome, this study selected the mavs gene as the target, designed sgRNA, constructed the recombined lentivirus expressing sgRNA of mavs, and infected the DF1-Cas9 cells.T7E1 enzyme digestion test and mavs gene targeting sequencing methods were used to analyze whether the technology can be used for gene editing of chicken cells.The targeting fragment of 1 220 bp was amplified, and two bands about 300 bp and 900 bp were observed by T7E1 digestion and agarose gel analysis, indicating that the cells infected with the lentivirus were mutated.Sequence analysis revealed that multiple base deletion mutations were present in the targeting sequences, indicating that CRISPR/Cas9 technology based on lentivirus system could be used to edit the chicken genome.