A novel high performance liquid chromatography-ultraviolet method (HPLC-UV) was developed for quantitatively determining the content of hazardous 3-chloro-1,2-propanediol (3-MCPD) in soy sauce.3-MCPD was first azidated with sodium azide,and the obtained azide product was further subjected to a CuAAC type click reaction with phenylacetylene,and the resulting derivative 3-(4-phenyl-1H-1,2,3 -triazol-1-yl) propane-1,2-diol (3-PTPD) was obtained and analyzed with HPLC-UV finally.The detection limits and quantitation limits of 3-MCPD were 0.1 and 0.3 μg/mL,respectively,and the linear relationship was good (r2=0.999).The results of spiked recovery test of soy sauce samples showed that the recoveries of the method were from 98.63% to 100.17%,and the relative standard deviation (RSD value) was from 1.95% to 4.74%.This method is compared with the national standard test method GB 5009.191―2016 as well,and the results obtained by the two methods are consistent.