Based on the transcriptome data of Dryopteris fragrans,the gene sequence of MYC2 transcription factor MYC2 was cloned and named as DfMYC2.The gene accession number was MK193871.Bioinformatics analysis of the protein sequence,fluorescence quantitative analysis of different parts and expression analysis of 0.4 mmol/L methyl jasmonate (MeJA) were performed.The results showed that the DfMYC2 gene was 3 130 bp in full length and the open reading frame (ORF) was 2 496 bp in length,encoding 831 amino acids with a relative molecular weight of 261 041.86.The leaves of D. fragrans were sprayed with 0.4 mmol/L MeJA solution,and samples were taken at 0 h,2 h,4 h,6 h,8 h,12 h,24 h and 36 h after treatment.The results of realtime quantitative PCR showed that DfMYC2 had the highest expression at 4 h after treatment.It will provide the foundation for further studying the biological function of DfMYC2 gene in D. fragrans