A PutNaKR3 gene was screened and identified from the cDNA library of Puccinellia tenuiflora under NaCl treatment. Bioinformatics, tissuespecific expression and abiotic stress response expression of the PutNaKR3 gene and its encoded amino acid sequence were performed. The results showed that PutNaKR3 gene in Puccinellia tenuiflora had a tissue expression specificity, and its expression in roots and leaves was significantly higher than that in other tissues. Overexpression of PutNaKR3 gene enhanced the viability of yeast strain under salt, alkali, oxidation and osmotic stress, and improved the adaptability of yeast under partial metal stress. It is indicated that the PutNaKR3 gene plays a certain role in plant stress response, especially in enhancing plant salt tolerance.