A total of 46 pomelo resources in Guangfeng County and its surrounding areas of Jiangxi Province were identified by SSR molecular marker technique to explore the genetic relationship and origin of Majia pomelo and its main pomelo lines around its origin，and to screen varieties with suitable pollination. The fruit quality-related indexes of Majia pomelo with different cross-pollination combinations were analyzed for two consecutive years. The results showed that Majia pomelo had obvious variation during the long-term seed reproduction and artificial selection. It is most likely derived from the local pomelo in Guangfeng and its surrounding areas through long-term natural and artificial selection. Among the different cross-pollination combinations，native pemolo No. 4 has the best effect on improving the fruit quality of Majia pomelo，which is the most suitable male parent for pollination at present.