“Huayouza 9” was used to study the differences in the growth and boron absorption and utilization of rapeseed with two different forms of boron including boric acid and diglycerol borate under water culture to explore whether organic boron （diglycerol borate） has the effect of boron fertilizer.The results showed that boron deficiency treatment led to thickening，curling of rapeseed leaves，and decreased biomass，while the application of organic or inorganic boron promoted the growth of rapeseed.Inorganic boron was more conducive to the growth of rapeseed than organic boron under the treatment with low boron （0.25 μmol/L）.Organic and inorganic boron had similar effects on the total biomass of rapeseed under the treatment with suitable boron （25 μmol/L）.Whether in a low boron or suitable boron environment，the content of photosynthetic pigments including chlorophyll a，chlorophyll b and carotenoids in rapeseed leaves after applying organic boron was significantly higher than that of rapeseed treated with inorganic boron.The photosynthetic rate of rapeseed after applying organic boron was significantly increased by 28.9% compared to applying inorganic boron under the treatment with suitable boron （25 μmol/L）.The accumulation of starch grains in leaves increased.The boron absorption per plant of rapeseed significantly increased by 11.7%，with the main increase being in the aboveground part of rapeseed.It is indicated that organic and inorganic boron have similar physiological functions in rapeseed under suitable boron environment.Organic boron is more conducive to the absorption，accumulation，and upward transportation of boron in rapeseed compared to inorganic boron，promoting the generation of photosynthetic pigments and thereby improving photosynthetic efficiency.