In order to explore the effects of N，K，Ca，Fe，and Zn and their interactions on cassava yield，nutrient content and expression of genes related to the absorption and transport of five nutrient elements，using the edible cassava variety SC9 with low hydrocyanic acid content as the test material，an orthogonal experiment of two-level fertilization with five factors of N，K，Ca，Fe，and Zn was designed under the conditions of pot culture and field culture.Differences of cassava yield，chlorophyll content，accumulation of large，medium and trace elements，and the expression of genes related to the absorption and transport of five elements were compared among the treatments，and the best application range of N，K，Ca，Fe and Zn was selected.The results showed that both pot and field T5 （N₁K₁Ca₂Zn₁Fe₂） treatments could obtain the higher cassava yield.High N could promote the synthesis of chlorophyll under pot conditions，but the application of high levels of macronutrients would significantly reduce cassava yield，while the application of high levels of micronutrients would increase both chlorophyll synthesis and yields under both conditions.Under field conditions，high N levels not only promoted chlorophyll synthesis but also increased yield.Regarding element accumulation，the average accumulation of N，P，K，Ca，Fe，Mg in roots of low N and low K treatments was significantly higher than that of high N and high K treatments； the average accumulation of P，K，Zn in roots of high Ca treatment were significantly higher than those of low Ca treatment； the average accumulation of N，K，Ca，Zn in roots of high Zn treatment was significantly higher than that of low Zn treatment； the average accumulation of P，K，Ca，Zn，Fe，Mg in roots of high Fe treatment was significantly higher than that of low Fe treatment.From the perspective of gene expression，high concentration of N，K，Fe，Zn，fertilizer upregulated the expression of MeNRT1，MeAKT1，MeVIT1 and MeIRT1 genes，while low concentration of Ca and Zn fertilizer upregulated the expression of MeCBL7，MeCNGC14 and MeZIP4 genes.In summary，different fertilization treatments have significant effects on the synthesis of chlorophyll content in plant leaves，root yield，root nutrient accumulation，and the expression of nutrient transporter related genes.The lack or excess of elements as well as the relative imbalance are not good for the yield and quality of cassava and we should pay attention to the application of reasonable proportion to maximize the function of elements.Finally，for cassava pot experiment，the suitable fertilizer application amounts were 0.280-0.560 g/kg of N，0.210 g/kg of K，0.165-0.330 g/kg of Ca，0.081-0.163 g/kg of Zn and 0.111 g/kg of Fe，and the suitable fertilizer application amounts in field experiment were 150 kg/hm² of urea，86 kg/hm² of potassium chloride，20 kg/hm² of calcium chloride，90 kg/hm² of zinc sulfate heptahydrate and 35 kg/hm² of ferrous sulfate.