This article used decomposed pig manure， pond soil， cottonseed lint， and other waste materials as the substrate for cucumber seedlings to study an efficient and affordable formula of substrate for growing the seedlings. The suitable formulas of substrate for growing the seedlings were selected by studying the physicochemical properties of different ratios of substrates and its effects on growing the seedlings. The results showed that the substrate made of decomposed pig manure， pond soil， and cottonseed lint as partial replacement of peat vermiculite （peat︰vermiculite=2︰1， the same below） was suitable for the physical and chemical properties of the substrate compared to the control， and promoted the growth of cucumber seedlings and the accumulation of dry matter. Among them， the ratio of peat vermiculite︰ decomposed pig manure︰pond soil︰ cottonseed lint=50︰20︰20︰10 was suitable for the physical and chemical properties of the substrate， with the highest comprehensive evaluation index and the best comprehensive performance of the seedlings. It will provide a technical support for the efficient utilization of wastes including pig manure， pond soil， and cottonseed lint in the compound substrates for cucumber seedlings.