Yunyan 87 was used to study the differences in the effects of spraying ordinary molybdenum fertilizer and nano molybdenum fertilizer on the growth of tobacco and the accumulation of nitrogen-phosphorus-potassium in flue-cured tobacco.A pot experiment with five treatments including control （CK）， ammonium molybdate （T1）， nano molybdenum oxide （T2）， nano zinc molybdate （T3）， and nano potassium molybdate （T4） was conducted to analyze the agronomic traits， the accumulation of dry matter， the content of molybdenum， and the accumulation of nitrogen-phosphorus-potassium in tobacco.The results showed that both spraying ordinary molybdenum fertilizer and nano molybdenum fertilizer effectively increased the plant height， leaf length， leaf width， and the content of total sugar in tobacco leaves， but the effect of the treatment with nano molybdenum zinc sulfate was the best.The fresh weight and dry weight of tobacco leaves under nano molybdenum zinc sulfate treatment increased by 32.29% and 29.95% compared to that under T1.The accumulation of molybdenum in tobacco leaves under the T1， T2， T3， T4 increased by 18.48 times， 17.30 times， 11.72 times， and 16.70 times compared to that under the control.The accumulation of nitrogen-phosphorus-potassium in tobacco leaves tended to increase under molybdenum fertilizer treatments.The accumulation of nitrogen， phosphorus， and potassium in tobacco leaves under T3 increased by 30.61%， 39.94%， and 48.78% compared to that under the control， surpassing the increase of 18.86%， 28.79%， and 20.92% under T1.It is indicated that nano molybdenum fertilizer is more effective than ordinary molybdenum fertilizer in promoting the growth of tobacco seedlings and the absorption of nitrogen， phosphorus， and potassium， with nano zinc molybdate （T3） having the best performance.