Rapid detection of 11 common pesticide residues in bananas with double-swab solid-phase microextraction combined with UPLC-MS/MS
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1.College of Food Science and Technology,Huazhong Agricultural University, Wuhan 430070, China;2.Analysis and Test Center, Chinese Academy of Tropical Agricultural Sciences, Haikou 570000, China

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O657.7

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    Abstract:

    A rapid and accurate method for detecting 11 common pesticide residues in bananas was established with solid-phase microextraction combined with ultra-high performance liquid chromatography tandem mass spectrometry (SPME-UPLC-MS/MS) to simplify the steps of detecting multi-pesticide residues in bananas, shorten the time of detection, and reduce the cost of detection. Swabs were used as support carriers to prepare the double extraction heads with broad-spectrum and large-capacity. The pesticides were extracted from diluted banana homogenate samples with the double extraction heads. Trace amounts of methanol were used to desorb pesticides from the heads, followed by detection with SPME-UPLC-MS/MS and quantification with method of matrix-matched external standard. The results showed that the limit of detection of 11 pesticides in bananas was 0.23-2.43 μg/kg, with the limit of quantification of 0.68-7.36 μg/kg. The rate of recovery at different spiked levels was 86.2%-109.8%, with a relative standard deviation of 0.6%-11.2%, which complies with the relevant regulations of detecting pesticide residues. It is indicated that the established method is simple, rapid, sensitive, accurate, and cost-low, and can meet the requirements for rapid detection of multi-pesticide residues in bananas.

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郑美杰,郑腾飞,刘春华,王明月,李秀娟. Rapid detection of 11 common pesticide residues in bananas with double-swab solid-phase microextraction combined with UPLC-MS/MS[J]. Jorunal of Huazhong Agricultural University,2025,44(4):310-317.

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History
  • Received:May 20,2024
  • Revised:
  • Adopted:
  • Online: July 24,2025
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