To investigate the effects of combined supplementation of sodium humate and Bacillus subtilis on growth performance, antioxidant capacity and immune function of Procambarus clarkii, juvenile crayfish were fed with 0 (CN), 0.5% Bacillus subtilis (BS), 0.5% sodium humic acid (HNa), 0.5% sodium humic acid and 0.5% Bacillus subtilis (BS+HNa) in commercial diets for 6 weeks. The results showed that the feed index of the diet supplemented group was significantly lower than that of the CN group, and the feed index of the BS+HNa group was the lowest, while the weight gain rate, specific growth rate and protein efficiency were significantly higher than those of the CN group (P<0.05). In terms of anti-oxidation, the activities of CAT and T-AOC in hemolymph and hepatopancreas of BS+HNa group were significantly higher than those of other groups, the content of MDA was significantly lower than that of other groups, and the level of SOD was significantly higher than that of control group (P<0.05). In terms of immune function, the activities of AKP and ACP in hemolymph and hepatopancreas of each supplemented group were significantly higher than those of the control group (P<0.05), and the activities of BS+HNa group were the highest. In conclusion, the addition of 0.5 %sodium humate and 0.5 %Bacillus subtilis can improve the growth performance, antioxidant capacity and immune performance of Procambarus clarkii, and the effect of combined addition is better than that of single addition. Therefore, it is recommended to use the combination of sodium humate and Bacillus subtilis.