Abstract:

Abstract:Cytochemical location of acid phosphatase of Robinia pseudoacacia L. seedlings inoculated with Glomus mosseae of arbuscular mycorrhizal fungi(AMF)were conducted. The results show that the observation of cytochemical location with electrical miroscope disc

Abstract:During a recent survey of fungi associated with bark beetle in Qinling Mountains of China, a new species was isolated,and the aim of this study was to describe this fungus.

Abstract:Research advances on freshwater fungi in Mainland China are reviewed, including their taxonomy, biodiversity and application. Since 1980, many new species records have been reported from Beijing, Anhui Province, Sichuan Province, and Yunnan Province. They

Abstract:Two species belonging to two genera of the Laboulbeniales were collected from Dermaptera in Korea. Among these species,Dimeromyces anisolabis Thaxter parasitic on Anisolabis maritima (Bonelli) is new to Korea and Filariomyces for ficulae Shanor parasitic

Abstract:Inhibitions of lysine analogue S-2-Aminoethyl-L-Cysteine(AEC) on myceliai growth of Pleurotus eryngii, Flammulina velutipes (IM02 ) , Pleurotus tuber-regium , Pleurotus citrinopileatus, Agaricus blazi and Agrocybe chaxingu (white) were reported. Myceliai

Abstract:对不同菌株、不同培养基和培养时间对金针菇菌丝体产量和金针菇多糖产量的影响进行了研究，并对金针菇胞外多糖进行了紫外和红外分析。结果表明，金针菇12号菌株在液体培养基中培养10～11 d时金针菇菌丝多糖含量和金针菇菌丝体产量最高，金针菇胞外多糖产量较高。紫外和红外分析结果表明，本研究所分离的金针菇胞外多糖为一种含β-型吡喃糖苷键的纯多糖。采用不同的培养基对金针菇胞外多糖的主体结构影响不明显，均有相似的多糖特征峰和β-型吡喃糖苷键吸收峰。但采用玉米、黄豆等配制的培养基更有利于工业化生产金针菇多糖。

Abstract:The solid-fermentation by Moanscus spp. with different substrate rice was investigated. The results showed that the content of Monacolin K was higher when the substrate rice was qualified with abundant proteins and carbohydrate, low C/N, the optimum whiti

Abstract:以三因素三水平的正交试验设计培养条件，接种量5%（V/V），pH=6.5，120r/min,28℃，120h，对蝉拟青霉菌Paecilomyces cicadae进行液体培养。通过对生长的菌丝体干重的测定及分析，得到该菌生长最适培养基配方为葡萄糖：20g/L，酵母膏：5g/L，K2HPO4:1g/L,并证明碳源为其生长的最重要营养因素。

Abstract:以洛巴伊口蘑（Tricholoma lobyense Heim)生物学特性做了初步的研究。结果表明，适于洛巴伊口蘑菌丝生长的最佳碳源为蔗糖，最佳氮源为酵母膏；最佳pH6.5,最佳温度为30℃；在黑暗状态下更适于生长。最佳自然基质为棉籽壳，最适宜的料水比为1∶1.40。

Abstract:报道了分离提纯自古尼虫草（Cordyceps gunnii）一个单子囊孢子的无性古尼拟青霉（Paecilomyces gunnii)5-19菌株，菌丝培养物镇痛组分是水溶性的；镇痛驵分最佳提取溶剂为水，最佳提取方法为热提取法；古尼虫草镇痛组分对冰醋酸所致小鼠扭体抑制率在剂量0.05g/g（生药/小鼠)范围与剂量成正相关；在所试pH值范围内（pH1～10），其镇痛效果无明显变化。当pH＞11时，镇痛作用强度下降。镇痛且分有较好的热稳定性。在100℃加热1h，它对冰醋酸所致的小鼠扭体抑制率仍为100％；培养基组成和培养温度在所试条件下，不仅影响菌丝的生物量，而且影响菌丝浸出物的镇痛效果。

Abstract:In this paper, the dynamic relationships between fermentation time and mycelium yield, reducing sugar content, total sugar content, pH value and amino nitrogen of Flammulina velutipes fermentation were investigated by submerged culture method, and the opt

Abstract:研究了不同生物反应器体系中丝状真菌产生γ-亚麻酸的工艺，结果表明：气升式发酵体系明显优于搅拌式发酵体系，二者γ-亚麻酸含量相差6％。并最终确定了气升式发酵体系下的工艺参数：pH6.0、通气量1∶1.7、发酵温度28℃，在此条件下γ-亚麻酸最终含量达到15％～18％。

Abstract:以8个银耳菌株为材料,研究交配型因子A在不同菌株中的分布,菌株内及菌株间单孢分离物配对试验结果表明,此8个菌株中只有两个不同的A因子,与前人报道的银耳是限制型双因子交配系统的报道是一致的.研究了培养条件对银耳孢子产交配信息素的影响, 结果表明银耳孢子产交配信息素的较佳培养基为 蔗糖 20 g/L ,(NH4)2SO4 1.584 g/L(含N 24 mmol/L), MgSO4*7H2O 0.25 g/L , KH2PO4*3H2 O 0.5 g/L , VB1 0.2 mg/L , ZnSO4*7H2O 2 mg/L , CaCl2*2H2O 0.5 g/L,钼酸铵0.02 mg/L.最佳发酵条件为培养基pH 6.5,23 ℃,140 r/min,在优化培养基和培养条件下,培养4 d发酵液中交配信息素的活性最高,可达到12 U/mL,是在基础培养基中活力的5～6倍.银耳交配信息素经胃蛋白酶处理后,活性完全丧失,由此推测,银耳交配信息素是一种多肽类物质.

Abstract:Gomphus purpuraceus is an important rare mycorrhizal edible fungus. It is very difficult to identify the isolates by fruiting. The tissue isolation from different parts of different developmental phases of fruiting body and mycorrhiza was made with 5 medi

Abstract:采用4种不同方法进行蛹虫草液体菌种的栽培试验,结果表明蛹虫草菌丝体在21～22 ℃时较27 ℃生长更好.观察了蛹虫草菌在8种不同的处理方式下对家蚕蛹的感染情况,推测高温条件可能破坏蛹体表皮的几丁质,使蛹虫草菌丝体更易于感染家蚕蛹.

Abstract:The cell long and wide of stipe were measured at different development stage of Volvariella volva-cea fruit body and the growth rate of different part of stipe was estimated by marked with ink. The result showed that the stipe cell began to elongate in eg

Abstract:The concentration of ABA and GA3 and ZR of stipe tissue were determined in the different development stage of the tiny button, the button, the egg, the elongation and the mature. The results showed that ABA concentration in the egg stage was the highest a

Abstract:Gastrodia elata Blume is a medicinal plant and widely distributed in Korea, China and Japan. The dried tuber of G. elata have been used as traditional herbal medicines for curing human diseases. Here, we reported the observation of developing procedure of

Abstract:比较了利用无菌短木接种法、钻孔接种法和端木夹心接种法等接种方法接种桑树段木后Phellinus linteus的菌丝生长和定殖状况.研究表明利用灭菌的短木接种P.linteus,生长和定殖良好,传统的钻孔接种法和端木夹心接种法菌丝生长定殖不好.P.linteus在20 cm长的桑木上生长和定殖所需的最适的含水量为42%,接种后12 h,P.linteus立即进入快速生长期.接种后的桑木埋入土壤中需要5～6个月产生担子果,在培养室内温度为31～35℃,相对湿度大于96%时有利于子实体的形成.

Abstract:探讨了一系列有关菇床的制备、栽培料的处理和发酵等提高平菇产量的栽培措施。为了提高锯木屑的利用率，在制备菇床时引进了发酵技术；为了解决生长中一些基础问题，改进了培养基质的处理方法。同时也评估了聚乙烯薄膜钻孔覆盖技术对两种平菇（Pleurotus ostreatus和P.sajor-caju)产量和品质的影响。在平菇菇床制备的过程中，为避免其它真菌菌丝的污染，使用了发酵后的锯木屑法。结果表明，所用的3种添加物中，稻糠是促使培养基质升温效率最高的添加物，可以升高70℃。应用发酵锯木屑添加稻糠生产的菌丝的活力和密度大大高于非发酵锯木屑在接种后30d内对木霉无能为力。研究了堆料（发酵）对平菇培养基物效率的提高能力。在白杨树锯木屑和冬蘑菇的栽培废料中加入10的米糠后，在20～28℃下室外堆放12d。在发酵锯木屑钵中发酵的持续时间缩短3d。平菇在发酵基质中生长可以加快子实体原基的形成。用棉籽壳和发酵的白杨锯木屑混合可以获得最高的产量（742g)，而对照只有663g。另外，用白杨锯木屑与冬蘑菇培养废料混合可以最有效提高发酵效率。在棉籽壳中添加发酵的锯木屑和可循环的农副产品可以促进培养基质发酵和子实体形成。许多问题，凿孔聚乙烯薄膜覆盖技术被用来栽培Pleurotus ostreatus和Pleurotus sajor-caju.菇水覆以打孔的、透明的、黑色的塑料膜（0.03mm厚，孔直径10cm，孔间距10cm以保证25孔/m^2)。菌种自孔接入菇床表面。此项技术因蘑菇成束形成而使采摘时效率更高，P.ostreatus的产量每平方米增加5.7％到10.8kg。聚乙烯薄膜覆盖的菇床每束蘑菇的平均重量283g(33子实体），而传统的栽培方法为117g(15子实体）。P.sajor-caju可以快速在基质上生长和定殖，并在孔口周围成束长出子实体。P.sajor-caju栽培系统的总产量为8kg/m^2，薄膜覆盖栽培平均每束菇的重量为225g（79个子实体，而传统的方法栽培的平均子实体产量为2.5g。由于菇床平坦，有利于快速采摘，降低劳动力达52％（P.ostreatus)或64％（P.sajor-caju）。在薄膜下面，平菇的菌丝保持健白色，健康状态，而传统的栽培经过一轮的采摘后，菌丝变黄。用薄膜覆盖技术很少出现菇体不能发育成熟的现象。因此这种建立在提高栽培料组合和发酵基础上的平菇栽培新方法值得推荐。

Abstract:The traditional medicinal efficacies of Ganoderma lucidum are treatments of hepatopathy, nephritis, hypertension, hyperlipidemia, arthritis, neurasthenia, insomnia, bronchitis, asthma, gastric ulcer, arteriosclerosis and diabetes. Some of these efficacies

Abstract:试验测定了8个粘帚霉Gliocladium spp．菌株的发酵液对3种重要土传病原真菌尖孢镰刀菌Fusarium oxysporum Schlecht．、立枯丝核菌Rhizoctonia solani和核盘菌Sclerotima sclerotiorum的抑菌效果。结果表明，HL-1-1、SH-1-1和SS-1-1的抑菌作用最强，抑菌率分别达83．48％、81．65％和78．68％，3个菌株之间差异不显著；SDT-lO-1的抑菌作用最小，抑菌率仅为23．24％。热稳定性测定结果表明：发酵液121℃处理30min，病原菌生长正常，抑菌作用消失；发酵液经60℃处理后供试菌株的抑菌效果明显，但效果明显降低。

Abstract:Jinggangmycin soluble powder(SP) is a new fungicide formulation. It is excellent for its high concentration, less dosage, low cost. But this new formulation has never before been reported. The author does a field trial for control of the rice sheath bligh

Abstract:The powdery mildews (Erysiphales) are very important parasitic fungi which cause powdery mildews on different economic plants. From 1999 to 2003,the flora and distribution of the powdery mildews of Gansu province have been studied,More than 500 specimens

Abstract:Based on the rust fungi collected from Korea during 1995~2001 period, 71 species of Pucciniaceae were identified. Host plants, herbarium numbers and location of collection sites were also recorded. Of these, 55 are Puccinia, 13 Uromyces, 2 Gymnosporangium

Abstract:Scolytid bark beetles (Coleoptera: Scolytidae), which are known to be associated with blue stain fungi(BSF) causing sap-stain in trees or freshly cut logs, were collected from the trap logs of different pine species; Pinus. rigida, and Pinus. koraiensis e

Abstract:We studied the relationship between the polymorphisms of the mycelial growth vigor and growth rate with the mating type of 38 sporulated monocaryotic strains, esterase patterns polymorphism of 14 sporulated monocaryotic strains, carboxymethyl cellucose(CM

Abstract:Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is a major endothelial receptor for oxidized low-density lipoprotein (oxLDL), and is assumed to play an important role in atherosclerosis. LOX-1 cD-NA was obtained from human THP-1 cells by R

Abstract:A novel negatively charged cordycepin-[Mg-Al-NO] nanohybrid has been prepared by ion-exchanged method. Results of TEM, PXRD and FT-IR spectroscopy confirmed that cordycepin could be intercalated into [Mg-Al-NO3] interlayers as the charge-compensating spec

Abstract:Monokaryotic strains H2 and J3 were respectively developed from the cultivated strains He-l and Ju-1 of Auricularia auricula through protoplasted monokaryon technique. Dikaryotic strain H2 J3 was bred through crossbreeding of H2 and J3 as parents. Fifty-t

Abstract:28S rDNA 5' half, ITS and IGS1 region of eighteen Pleurotus taxa, together with Agaricus bis-porus, Lentinula edodes and Hohenbuehelia serotina were amplified using PCR and then digested with seven restriction endonucleases. A single uniform 1. 46 kb prod

Abstract:Chitosanases catalyze the hydrolytic degradation of chitosan. They are important for the extensive carbon and nitrogen recycle which occurs in nature and the industrial application which are sea-food-processing and size-specific chitosan oligomer producti

Abstract:The white-rot basidiomycete Trametes versicolor produced high levels of Mn-peroxidase activity when grown on Kirks media with 0. 05% Tween 80, 2. 4 mmol/L (NH4)2-tatrate, 80 mg/kg of MnSO4. Maximum activity of Mn-peroxidase was appeared on the 10th day of

Abstract:在韩国的平菇(Pleurotus ostreatus )商品化种植基地发现了真菌病毒的自然流行为害的现象。这些病害经常伴随着一些球形病毒粒体的出现。采用Tris-EDTA缓冲液提取，PEG-NaCl沉淀和CsCl梯度离心，依据不同的比重，获得了3种病毒粒体，其中1种病毒粒体直径为27nm，另外2种的粒体为27nm，并推定这些病毒共同感染栽培的平菇。其中1种27nm的球形病毒，其比重较34nm的病毒大，称为平菇球形病毒(OMSV)，该病毒含有2条大小分别为6．00kb和1．25kb的ssRNA片段。直径为34nm的病毒有两种比重小的粒体，其中1种常与OMSV共感染平菇，称为平菇等面体病毒-I(oystermushroom isometric virus-I ,OMIV-1),该病毒含有12条dsRNA片段，它们的大小分别是2.65，2．45，2．40，2．20，2．15，2．10，2．05，2．00，1．90，1．80，1．00和O．80kb。另外一种称之为平菇等面体病毒-II(oyster mushroom isometric virus-II, OMIV-II),．含有3条dsRNA片段，大小分别是2．25，2．15和2．05kb。经聚丙烯酰胺凝胶(12％)电泳、考马斯量兰染色表明OMSV，OMIV-I和OMIV-II病毒粒体的外壳蛋白的分子量大小分别是29kDa、71kDa和62kDa。这些结果表明在平菇中至少存在3种不同的ssRNA和dsRNA病毒。

Abstract:自韩国平菇生产地分离到26株木霉(Trichoderma)，基于培养特性和菌落形态，这些菌株分为4组，鉴定为Trichoderma sp．K1、Trichoderma sp．K2、T．harizianum，和T．atroviride，其中优势种为Trichoderma sp．K2，其次是Trichoderma sp．K1和T．atroviride。K1和K2不仅在形态上与其它的组有区别，而且相互在生长温度(35℃)，菌落形态，分生孢子的形态，分生孢子小梗及其分枝类型，分生孢子的形态等方面有显著差异。对这些菌的ITS、EF-1(和RPB2的序列比较显示出这2个待鉴定的种(K1和K2)不仅与早期报道的木霉种有差异，而且相互间也显著不同。同时发现，EF-1(和RPB2序列比ITS序列在木霉的系统分类上更加客观可靠。基于以上研究结果，我们认为K1和K2是木霉属中的新种，即将给予它们新的名称。

Abstract:Cordycepin has a variety of biological activity.It can inhibit synthesis of DNA and RNA in cancercells, can enhance cell differentiation, restructurecell cytoskeleton, inhibite protein kinase activityand so on. It even has antitumor activity on blad-der, kidney as well as lung, can inhibit infection

Abstract:In the 1970's, a Japanese scientist discovered that some strains of Monascus spp. when they grew on rice might produce a certain secondary metabolite, known collectively as Monacolin K,

Abstract:Paecilomyces is a genus closely related toPenicillium. Most species of Paecilomyces arecommon soilborne saprobes, a section in this genusis known primarily as parasites of insects. Somespecies of another section are quite tolerant of hightemperatures.

Abstract:天然产物紫杉醇是一种重要的抗癌药。其天然来源的匮乏和缺少商业上可行的化学全合成促进了对紫杉醇生物来源的深入研究。紫杉醇的生物合成是一个复杂的多步过程，主要包括四环骨架的构建和加入各种羟基和酰基基团，其中羟基的加入由细胞色素P450氧化酶催化。我们从中国红豆杉愈伤组织细胞mRNA构建的cDNA文库中克隆得到几个P450 cDNA片段。其中一个长1494bp的cDNA片段，编码497个氨基酸，推断蛋白分子量为56470 Da，等电点为9．42。序列比较显示，这个推断蛋白包含多个细胞色素P450氧化酶的保守区，具有典型的细胞色素P450氧化酶的特征，进一步的比较发现其与已鉴定的东北红豆杉紫杉烷-10β-羟化酶有92％的同源性。将这个cDNA片段连接在质粒pYeDP60上构建表达载体，导入相应的酵母表达菌株WHT和WVS进行表达，获得相应大小的蛋白表达条带，功能鉴定的工作正在进行中。