Abstract:By use of TAC vector(transformation-competent artificial chromosome) pYLTAC7, 35 kb genomic DNA of Minghui 63 was transformed into Nongken 58S through Agrobacterium-mediated method, through which an attempt was made on the availability of pYLTAC7 Vector i

Abstract:采用离心、超滤、DEAE-Sepharose FF阴离子交换层析柱等方法对稻瘟菌(Magnaporthe grisea)细胞壁来源的激发子活性物质进行了提取；以一套水稻抗稻瘟病近等基因系为材料，对其诱导水稻叶片植保素、过氧化物酶(POD)与苯丙氨酸解氨酶(PAL)活性进行了检测；结果表明，粗激发子提取物(CEF)及DEAE-SepharoseFF层析提取物DSⅡ和DSⅢ菌具有激发子活性。经热、胰蛋白酶和过碘酸钠处理后的生物活性检测结果表明DSⅡ和DSⅢ的活性位点均位于糖基部分。

Abstract:通过盆栽试验研究了包膜尿素(自制)和尿素等肥料在灰潮土中对芹菜生长、氮吸收、产量和品质等的影响。结果表明：在2种质地的灰潮土中施包膜尿素较尿素使芹菜增产11．5％～15．2％，N吸收增加5．9％～9．5％，土壤氨挥发损失N减少12．2％～14．4％，淋失和反硝化N损失减少25．5％～28．3％，N素利用率提高20．1％～27．9％，土壤持留N量占施氮量的32．0％～37．3％；芹菜产品中NO3^--N含量降低44．2％～60．3％，维生素C含量显著提高，品质明显得到改善。

Abstract:The soil physical and chemical properties were studied in fertile soil applying wheat straw with high ratio of carbon and nitrogen under the condition of cultivating flue-cured tobacco in Yuxi Yunnan Province. When 7 500 kg wheat straw was applied in 1 hm

Abstract:The influences of Mn toxicity on the growth, the content of Mn, Fe, Ca and the activities of POD and CAT in rape (Brassica napus L.) were studied under solution culture. The Mn content in different parts of rape were significantly increased under the cond

Abstract:The balanced fertilization technique on the growth of Phyllostacys praecox f. prevelnalis in Xianning City of Hubei Province was studied.The results showed that proper fertilization can improve the nutrient situation of soil, develop the growth of the ro

Abstract:利用绿色荧光蛋白基因gfp标记研究了土壤中Cu2+胁迫对饭豆(Vigna umbellate L.)根瘤菌JMC1402G腐生存活和共生固氮能力的影响.研究结果表明在液体培养条件下,向培养基加入1μg/mL的Cu2+时,JMC1402G的生长受到轻微抑制;当Cu2+浓度达到5μg/mL和7 μg/mL时,JMC1402G的生长受到严重抑制;当Cu2+浓度达到10μg/mL时,JMC1402G已不能生长.在灭菌土壤中Cu2+添加量小于200 mg/kg时,JMC1402G数量在37 d内能保持在1.74×105cfu/g以上,当土壤中Cu2+添加量达到500 mg/kg时,在接种后第2天其数量已降至102cfu/g以下.在未灭菌土壤中,当Cu2+添加量在0～80 mg/kg范围内时,JMC1402G的数量在接种后23 d已由108cfu/g降至104～105cfu/g,随着Cu2+添加量的进一步增大,其菌数迅速下降到104 cfu/g以下.饭豆盆栽试验结果表明Cu2+添加量在0～80 mg/kg范围内对JMC1402G共生固氮的影响并不大,当土壤中Cu2+添加量达到120 mg/kg时,对JMC1402G产生较明显的生理毒性,当Cu2+添加量达到300mg/kg时,JMC1402G失去结瘤能力.

Abstract:The mycelia growth state, the agronomic characteristics of fruitbodies and 15anti-hybrid ability and physical condition of seven different types of Hericium erinaceus strains were compared. The results of the screening tests showed that the strains of hig

Abstract:选择健康 35周龄新罗曼商品代蛋鸡 76 8只 ,采用 2× 4因子试验设计方法 ,分别在饲粮中添加 0 %、3%、5 %、7%的苜蓿草粉和 1.6 %和 2 .4 %的油脂 ,试验期 6 0d ,观测紫花苜蓿草粉和油脂对产蛋鸡生产性能、蛋品质及蛋黄颜色的影响。结果表明 :(1)添加苜蓿草粉和油脂后 ,对产蛋率、采食量、蛋重、料蛋比均无显著影响。从添加草粉对蛋鸡生产性能的影响看 ,饲粮中以添加 5 %草粉最为适宜。 (2 )添加苜蓿草粉和油脂后 ,蛋形指数、蛋黄指数、蛋壳相对重均无显著差异 ,但蛋壳厚度、哈夫单位有较大增加 ;添加苜蓿草粉对产蛋鸡蛋品质的影响优于油脂。 (3)添加苜蓿草粉组的蛋黄颜色均极显著高于不添加组 ,且随着添加量的增加 ,蛋黄颜色呈上升趋势 ;随着添加时间推移 ,到 30d时蛋黄颜色趋于稳定。添加油脂对蛋黄颜色无显著影响。 (4)添加苜蓿草粉后 ,经济效益显著提高。故结合生产实际 ,建议在产蛋鸡饲粮中添加 5 %的苜蓿草粉为宜。

Abstract:将自然发病的肺动脉高压综合征艾维茵商品代肉鸡按日龄分组，右心导管法测定平均肺动脉压(PAPm)，根据腹水量确定各组内腹水程度，设无腹水的正常鸡作对照．并进行显著性比较。结果：(1)24、36日龄组发病鸡的PAPm均极显著高于同日龄的正常鸡，30日龄组发病鸡显著高于正常鸡，42日龄组发病鸡高于正常鸡(8．3％)但无显著性差异。(2)发病鸡的PAPm在30日龄时极显著高于24日龄；36日龄时显著高于30日龄；40日龄时低于36日龄(4．3％)，但差异不显著。(3)发病鸡的PAPm与腹水量间呈极显著正相关。结果表明，PAPm在本病发生和发展过程中都起着重要作用，并且与本病致死率有一定联系。

Abstract:探讨了获取受精卵的时间、显微注射和培养液等因素对显微注射胚胎体外发育的影响。结果表明：在注射HCG后24～26h和18～22h时获得的受精卵的囊胚率分别为45％和20％，二者差异显著；注射胚和非注射胚卵裂率差异显著(56／80 VS 74／76)，但显微注射对2-细胞以后阶段的发育没有显著影响；mWM1培养液和mWM2培养液都能有效克服受精卵体外发育阻断。mWM2培养液更支持早期转基因胚胎体外发育。

Abstract:用经福尔马林灭活的哈维弧菌 (Vibrioharveyi)菌苗对大黄鱼 (Pseudodciaenacrocea)幼鱼进行浸泡免疫接种 ,分别以 1.0× 10 8cells/mL、1.0× 10 7cells/mL和 1.0× 10 6cells/mL的菌液浓度 ,浸泡处理鱼体 1min、10min和 6 0min后 ,放入设置在象山港内的网箱中饲养 ,并且在浸泡免疫接种后的 12 6d内 ,定期检测试验鱼的免疫保护力 (RPS)。结果表明 ,在浸泡免疫接种后的 5 6～ 77d内 ,受免鱼的RPS达到了 95 %～ 10 0 % ,随后RPS稍有下降 ,但是 ,直到免疫浸泡接种后第 112～ 12 6天时 ,供试鱼的RPS仍达到 70 %～ 80 %。从设置在野外的网箱中供试鱼的统计结果看 ,与对照组相比 ,免疫鱼疾病发生较少 ,发病时期推迟 ,病程缩短 ,成活率提高 (80 .0 %～95 .0 % ) ,明显高于对照组。对不同日龄大黄鱼幼鱼进行浸泡免疫的试验结果表明 ,4 0日龄、75日龄和 10 0日龄的大黄鱼幼鱼均能在免疫接种后获得特异性免疫保护力。在第 1次免疫接种 5 6d后以同样方式加强免疫 ,试验鱼在 112d内保持了很高的RPS水平 ,明显地高于同期的 1次免疫接种鱼体。

Abstract:By 4 kinds of index to analyze the food habits of Pseudorasbora parva in Liangzi Lake, the result indicated that the value of occurrence (97.06%), number (99.91%) and the index of relative importance (IRI=0.98) of the Phytoplankton was the highest, and we

Abstract:白斑狗鱼(Esox lucius Linnaeus)隶属鲑形目、狗鱼亚目、狗鱼科、狗鱼属，分布于亚洲、欧洲以及北美，我国仅产于新疆北部额尔齐斯河流域及附属流域水体，是新疆的主要经济鱼类之一。白斑狗鱼肉质坚韧少刺，肉味鲜美，营养价值高，可食部分大，是深受人们喜爱的水产品。过去一直依靠天然捕

Abstract:以贮藏性有明显差异的黄花梨和湘南梨为试材，研究果实发育过程中钙调素(CaM)含量的变化。结果表明：梨果实生长曲线为非典型的S型，湘南梨果肉CaM在果实发育前期含量较高，果实迅速膨大期CaM降至果实发育的波谷，果实采收前CaM出现峰值，采收时又下降。在果实迅速膨大期前，黄花梨果肉整体水平低于湘南梨，果实膨大期降到波谷，成熟前迅速升到峰值。梨种子发育曲线为典型的S型，盛花后50d左右，种子即将进入迅速生长期，CaM含量出现峰值，2个品种峰值差异大。

Abstract:Using C. crenata Sieb. et Zncc. as scion and Castanea mollissima Bl. as rootstock, the relationships between rootstock-scion compatibility and absolute value of their phenol content was studied. The results showed that the rootstock-scion compatibility is

Abstract:立地指数表是评定林地生产力高低的数量指标，在世界各国的林业生产中已得到广泛应用。对于同一树种(品种)，立地指数表的应用精度在不同的地区常因地域性的差异而受到限制。湖北省自20世纪70年代引种南方型杨树获得成功后，全省进行了大面积的南方型杨树的栽培造林。但至今为

Abstract:The new bamboo culm growth after mother trees planted for bamboo shoots forest of Phyllostachys praecox f. preveynalis have been studied. The results show that the number of new culms, diameter of breast-high, tree height, height of the culm with no branc

Abstract:A method for establishing the precise 3 dimension models of wildhaber-Novikon gear by the parameter modeling function of Pvo/E was presented in this paper. Firstly, the tooth profiles on the normal plane drawn and shift it to the transverse plane,second,i

Abstract:By the example of designing this paper put forward the method to design the construction of dehumidifying room of dehumidifing and cooling system for greenhouse under the high temperature and high humidity condition.The results of the experiment show that

Abstract:This paper analyzed the structure and the working process of three-layer-cylindrical grader and the grading performance and screen mesh-size are experimentally studied.The results indicated that the rotating speed has obvious effects on grading performanc

Abstract:介绍了“粮食产量综合预测系统”软件的设计思想，系统结构，功能模块。同时也扼要地介绍了预测因子的处理算法，气象预测模型、社会经济因子预测模型、气象和经济因子组合的预测模型以及多预测方案的集成预测模型在计算机软件中实现的方法。

Abstract:The paper discusses how to make use of VFW real-time video to implement precision focus conveniently, adjustde by manual work and distinguish by computer .It offers the strong means the capture and processing of Image measure system.

Abstract:This article summarized methods of pear meristem culture, and effects of different phytohormone kinds and concentrations, different culture media and conditions on surviving rates of pear in vitro plants. The application situation of pear meristem culture

Abstract:This paper summarized the environmental behaviors of sulfonylurea herbicides in soil, which included absorption, degradation and mechanism . The influences of the soil factors ,such as soil temperature, pH , water content, and organic matter were also dis