Abstract:To screen barley varieties suitable for genetic transformation that is not constrained by seasonal restrictions in field,fifteen elite barley varieties from China were compared for their callus induction and differentiation derived from mature embryos on

Abstract:以杂交中稻红莲优6号为材料,采用小区试验,研究了不同施氮水平和栽插密度及栽插模式对杂交中稻群体结构的影响。结果表明:杂交中稻红莲优6号在适宜的密度(2.7×105穴/hm2)和施氮水平(纯氮210 kg/hm2)下,采用宽行窄株的栽插方式,可以增加群体叶面积指数,提高光合速率,改善群体光环境,从而达到增加产量的目的。

Abstract:Some experiments revealed that the Ca2 has a relationship with drought resistance.In this paper,some physiological and biochemical indexes were studied in order to analyze the effect of Ca2 treatment on the drought resistance on Brassica napus.The physi

Abstract:为了探讨半夏试管珠芽形成的生理生化机制,以半夏无菌叶柄为材料,倒置在MS基本培养基上,对半夏珠芽形成过程中5种内源激素(IAA、GA3、ABA、ZR、JA)的动态变化进行了研究。结果表明:倒置半夏叶柄的珠芽无一例外地出现在叶柄形态学上端;ELISA方法测定显示,半夏叶柄形成珠芽的过程中叶柄上端IAA、ABA、ZR和JA的含量呈上升趋势,GA3在的珠芽形成初期急剧下降。随着珠芽的形成,GA3与其它各激素的平衡关系发生变化,IAA/GA3、ABA/GA3、ZR/GA3和JA/GA3的比值先呈下降趋势,15 d后开始上升;在珠芽快速形成期(10~20 d)IAA/GA3、ABA/GA3和JA/GA3维持在高的水平,其中JA/GA3的比值20 d后又呈上升趋势。

Abstract:记述采自中国广西壮族自治区蚱科蚱属Tetrix Latreille昆虫1新种,即钟山蚱Tetrix zhongshanen-sissp.nov.。模式标本保存在陕西师范大学动物研究所标本室(1♀)和广西河池学院动物标本室(1♀)。

Abstract:In this paper,the faunal structure of Chinese Elateridae was analysed based on the Elateridae samples in Institute of Zoology,Chinese Academy of Science,State Key Laboralory for Biocontrol,Sun Yat-sen(Zhongshan) University,Department of Applied Biological

Abstract:The amplification,cloning and sequencing of the rDNA-ITS region of 16 different populations of root-knot nematodes from China were conducted.The sequences blast results showed that six populations of root-knot nematodes on balsam pear,cowpea and rose coll

Abstract:采用药膜法、滤纸药膜选择法和三角瓶药纸熏蒸法分别测定了石菖蒲根茎提取物β-细辛醚(β-asa-rone)对谷蠹(Rhizopertha dominicaFab.)成虫的触杀、驱避及熏蒸作用。结果表明,β-细辛醚对谷蠹成虫具有较好的触杀与熏蒸活性。药膜法159.20μg/cm2浓度触杀处理72 h后对试虫的校正死亡率为100.00%。滤纸药膜选择法157.30、78.65、39.33、19.66μg/cm2剂量处理72 h内的平均驱避率均≥Ⅳ级。三角瓶药纸法166.67μL/L、83.33μL/L熏蒸处理72 h后试虫的校正死亡率分别为98.20%、75.86%。

Abstract:Based on the investigation of soil fertility of Jiangling County,Hubei Province,this paper compared the changing characteristics of the soil fertilities between 1982 and 2003.Results indicated that the area percentages of soil with high or very low organi

Abstract:对标准的链霉菌接合转移方法进行优化建立起适用于吸水链霉菌NRRL5491的接合转移系统。40℃温浴20 min为孢子的最佳热激处理条件;整合型质粒pSET152的接合转移效率是穿梭型质粒pOJ446的3~6倍;当阿泊拉霉素覆盖浓度为1μg/mL时,pSET152的接合转移效率是50μg/mL时的4.6倍,而pOJ446接合转移效率相应提高了2.4倍;MS培养基上接合转移效率是SY琼脂培养基上的3.4倍。采用最适优化条件时,接合转移频率可达到2×10-6,足够用于基因置换等遗传操作。

Abstract:原生质体制备与融合是重要的细胞工程技术,有利于实现远缘遗传物质的直接交换,促进遗传资源创新。本试验用Olympus BHS显微镜观察了曲霉原生质体融合全过程;采用淀粉-麸皮-硫铵培养基、淀粉-麸皮-豆粕培养基、马铃薯蔗糖琼脂培养基(PDA)等研究曲霉融合子及亲株产淀粉酶能力的特点;比较分析了亲株和融合子产淀粉酶性能与温度稳定性;比较研究了曲霉亲株和融合子淀粉酶的淀粉-聚丙烯酰胺非变性垂直平板凝胶电泳特征和各菌株可溶性蛋白质的淀粉-聚丙烯酰胺凝胶电泳特征。结果表明:曲霉原生质体融合过程很快,整个融合过程在5 min之内完成;融合子及亲株产淀粉酶随培养基、培养时间不同而变化;F05融合子在淀粉-麸皮-豆粕培养基产酶水平达(61 111.42±123.30)U/g,比亲株M70和M4分别高204.81%和114.00%,耐高温性能也有所提高;2种电泳结果都能够特异性显示淀粉酶带、重复性好。可见,原生质体融合是改良曲霉淀粉酶的有效方法。

Abstract:采用体外法研究了不同精粗比底物下(A组为全粗料,B组3∶7,C组5∶5,D组7∶3,E组为全精料)瘤胃真菌发酵特性及其对粗饲料(稻草秸)的附着情况。发酵试验结果表明:随底物精料比例的增加,同一时间点真菌数量呈上升趋势,A组到E组发酵96 h的累计产气量显著上升,分别为177.2 mL、198.2 mL、217.4 mL2、33.4 mL和251.5 mL,提示底物中精料比例的增加能促进瘤胃真菌的生长和发酵。此外,随底物精料比例的增加,羧甲基纤维素酶和α-淀粉酶酶活呈上升趋势,木聚糖酶酶活则呈下降趋势。稻草秸附着结果表明:不同发酵时期底物精粗比对附着于稻草秸的瘤胃真菌数量有很大影响。发酵前期(24 h),精料组(B组、C组和D组)附着于稻草秸表面的真菌孢子囊数显著高于粗料组(A组)。发酵后期(72 h),A组和B组附着于稻草秸表面和切缘的孢子囊数高于C组和D组,A组和B组附着于稻草秸表面和切缘的孢子囊数与48 h相比无显著变化,而C组和D组附着于稻草秸表面和切缘的孢子囊数显著下降。这种附着差异可能与不同精粗比底物提供的能量和附着位点不同有关。

Abstract:Eighty-four healthy Duroc Landrace Yorkshire barrows(10 kg body weight) were allocated to control(NRC.1998,nutrient requirements of swine) or treatment group(GB 7223-1987,nutrient requirements of Rongchang swine),each group having 6 replicates comprising

Abstract:The 151 samples of feed for ruminant were collected.The use of near reflectance spectroscopy(NIRS) was investigated as an alternative method for predicting chemical composition.The NIRS calibration models were developed by using the partial least squares(

Abstract:An indirect inhibiting ELISA method for the quantification of 11S(glycinin) and 7S protein(beta-conglycinin) was developed.First,soybean 11S globulin and 7S conglycinin were separated successfully by acid precipitation and affinity chromatography using a

Abstract:Feeding ability and growth of larval Misgurnus anguillicaudatus were investigated under artificial conditions.The results showed that larvae began to feed at 3 DAH(days after hatching).The yolk was depleted completely at 6 DAH.At 8~10 DAH,the unfed larvae

Abstract:研究了液相色谱法测定中华绒螯蟹组织中诺氟沙星、环丙沙星和恩诺沙星残留的前处理方法。结果表明:以提取前用无水硫酸钠作脱水剂、酸化乙腈作提取剂振荡提取、正已烷去脂、旋转蒸发并离心的前处理方法,灵敏、快速、准确、经济、实用性强。诺氟沙星、环丙沙星和恩诺沙星的最低检测限分别为5.0μg/kg、1.0μg/kg、5.0μg/kg;在加入标准品水平分别为5.0~15.0μg/kg、1.0~5.0μg/kg和5.0~15.0μg/kg时,回收率分别为68.67%~82.89%、66.67%~74.83%和72.67%~81.33%,相对标准偏差分别为1.22%~7.32%、5.19%~8.18%和2.52%~5.73%。该前处理方法适合检测诺氟沙星、环丙沙星和恩诺沙星残留。

Abstract:为摸索适宜椰子的SSR反应体系,分析了PCR反应体系中的Mg2 浓度、引物浓度、dNTP浓度、TaqDNA聚合酶浓度、模板浓度以及退火温度对扩增结果的影响,建立了适合椰子的SSR反应体系。研究结果表明:在20μL反应体系中,Mg2 、引物和dNTP的最适浓度分别为2.5 mmol/L、0.3μmol/L、0.2 mmol/L;TaqDNA聚合酶的最佳使用量为1 U,模板DNA应加入50 ng,引物最佳退火温度比Tm值较小者低2~3℃。在参数优化的基础上,应用标记分析该反应体系对24个海南不同地区高种椰子样品进行SSR分析,不同样品间DNA谱带多态性丰富,本研究建立的分析体系将为今后椰子资源的SSR分析奠定良好的研究基础。

Abstract:The inducing,subculture,and control browning of callus in leaves of Nubiana were studied in order to establish the optimum condition of inducing and growth of callus.The results showed that WPM medium supplemented with BA 0.5 mg/L and 2,4-D 2.0 mg/L was t

Abstract:利用包含双元载体pBI121的农杆菌LBA4404转化西瓜子叶外植体,获得转基因西瓜植株。结果表明:转化受体材料西瓜品种早花、克伦生、黑美人母本F23的分化效率没有明显差别;外植体浸染菌液10 min的处理较为合适;125 mg/L卡那霉素和300 mg/L羧苄青霉素适合于外植体筛选培养。通过PCR检测获得了3株转基因植株,转化效率为1.5%,其中只有2株整合了gus报告基因。Southern杂交和gus染色检测证明2株的基因组中整合了外源基因,报告基因在植株T0-1-3后代呈现3∶1分离。

Abstract:The leaves of Corylus plants are rich in secondary substances as polyphenols and polysaccharides which greatly decreased the quality of genomic DNA during DNA extraction.A procedure for DNA extraction from several species of the genus Corylus in China was

Abstract:研究了不同培养基、采集时间、贮藏温度和湿度等因素对百日草花粉离体培养萌发效果的影响。结果表明:花粉萌发较适宜的培养基为ME3 16%PEG4000 12%蔗糖;上午10:00采集花粉萌发率要显著地高于下午3:00采集的;在4℃湿润条件下贮藏18 h,J6花粉生活力下降较小,而在室温干燥条件下几乎失去活力;相对湿度在66%以下时极不利于花粉保存;11个百日草自交系花粉离体萌发率差异显著。

Abstract:In this study,stem from three years old Chinese toon from Xiaogan was used as material to study the tissue culture and regeneration system in Toona sinensis.The results are as follows:the solution of 0.1% HgCl2 was efficient for surface sterilizing of the

Abstract:半支莲(Portulaca grandifloraHook)为马齿苋科马齿苋属一年生草本花卉,又称松叶牡丹、太阳花、洋马齿苋,民间俗称“死不了”。由于植株低矮、花色丰富、花期长,且抗旱性强、病虫害少、耐粗放管理,既可作为地被植物,也可作为花坛、花境植物广泛应用于城市绿化中[1]。而且,半支莲全草均可入药,现代医学证明,半支莲有清热解毒,消肿止痛、活血化瘀的功效[2]。因此,无论从观赏应用还是从药用价值来考虑,半支莲都很有研究价值。半支莲育种的主要目标着重于丰富花色、花型,增加花数,增大花径,延长花期,降低株高,改善株型,增强抗寒性以及培育全日…

Abstract:以白鲢鱼骨蛋白为对象,在确定复合蛋白酶(protamex)适宜水解条件的基础上,采用正交试验对复合蛋白酶与风味蛋白酶(flavorzyme)分步水解白鲢鱼骨蛋白的工艺进行优化。结果表明,复合蛋白酶的适宜反应温度为50℃,适宜反应pH值为6.5,最适加酶量为3 000 U/g。采用复合蛋白酶和风味蛋白酶分步水解鱼骨蛋白可以提高水解度和氮收率,最适水解条件是在料液比1∶4(W/V),起始pH 6.5,用1 500 U/g复合蛋白酶于50℃下水解2 h后,再用1 500 U/g风味蛋白酶继续水解2 h,其水解度和氮收率分别为28.75%和79.69%,比单独用复合蛋白酶或风味蛋白酶水解时,水解度分别提高11.07%和9.81%,氮收率分别提高5.17%和13.22%。

Abstract:以白鲢鱼糜为原料,在确定凝胶劣化温度的基础上,研究了食盐、水分和可溶性蛋白对凝胶劣化的影响。结果表明,在供试温度范围内(40℃、50℃、60℃和70℃),随着加热时间的延长,鱼糜凝胶的破断强度呈现先增加后下降的趋势,且加热温度越高破断强度达到最大值所需的时间越短、下降也越显著。鱼糜凝胶劣化温度段为50~60℃,凝胶劣化与该温度段肌球蛋白重链的降解密切相关;盐溶蛋白和水溶蛋白都能不同程度地抑制凝胶劣化,但超过一定添加量时就会产生相反的作用;凝胶劣化程度随含水量的增加而增大,也随食盐含量(1%~3%)的增大而增大。

Abstract:Monopterus albus slices(mud slices) treated or not treated by 2.0% sucrose,2.5% salt were packed with common package,vacuum package and CO2 filling gas package,respectively,and then stored at control freezing-point temperature.The aerobic bacterial counts

Abstract:以毛棉籽油为原料,探讨了制取生物柴油过程中水洗法脱磷除胶前处理的最佳工艺参数。通过正交试验得出影响水洗过程毛棉籽油中胶溶杂质去除率的因素主次顺序为:水洗次数>水化时间>水化温度>搅拌速度>加水量。最佳工艺参数:水化温度60℃,搅拌速度150 r/min,水化时间15 min,加水量4%,水洗次数为3。磷脂的去除率达68.23%。

Abstract:利用高效液相色谱(HPLC)法同时分离测定了龙眼肉中的9种核苷类成分(尿嘧啶、胞苷、尿苷、胸腺嘧啶、次黄嘌呤核苷、鸟苷、胸苷、腺嘌呤相对标准偏差和腺苷)。分离柱为Nucleosil C18反相柱(250 mm×4 mm),流动相为0.01 mol/L的KH2PO4和甲醇,梯度洗脱,流速1 mL/min,检测波长260 nm,柱温40℃。用外标法测定龙眼肉中9种核苷成分的含量。在供试条件下,选取9种核苷标准品的线性范围为0.05~3.20μg/mL,相关系数r=0.9999~1.0000;标准品在0~48 h内经多次测定,保留时间和峰面积的相对标准偏差分别为0.133%~1.156%和1.153%~3.023%;回收率和回收率相对标准偏差分别为98.79%~107.34%和1.411%~4.544%;仪器最低检出限分别为0.0035~0.0136μg/mL。

Abstract:Based on the routine biochemical separating techniques,using RP-HPLC and electro-elution,a 20 ku antibacterial peptide had been purified from Musca domestica dry larvae detected by a highly sensitive bactericidal method.The peptide has bioactivity against

Abstract:Phytases play an important role in animal,plant and microbial.In recent years,with fungal genetic engineering and plant genetic engineering development,more than 10 fungal phytase genes have been cloned,and the microbial phytase production efficiency has

Abstract:Sulfur deposition at regional scale has been paid more and more attention to with accelerated acid deposition or acid rain worldwide.The study on sulfur deposition at regional scale has been initiated in developing countries.The sulfur deposition at regio

Abstract:Immune activation reduces animal growth performance by multiple cytokines'suppressing animal's appetite and catabolizing proteins and saccharides.Antibiotics can modify immune activation by its effect on activities of immune cells.Studies suggest that in

Abstract:Nitrogen metabolism in the rumen can be divided into 2 distinct events:protein degradation and microbial protein synthesis.Protein degradation in the rumen is the result of microbial activity and affects by physical and chemical characteristics of protein